October 25, 2025: We are pleased to announce the release of our new web server: NESPript It has been designed for the representation of multiple alignments of nucleic acid sequences, including the visualization of their secondary structures.
ESPript, Easy Sequencing in PostScript, is a web server that renders protein sequence similarity and secondary structure information from aligned sequences for analysis and publication purposes.
A comparable service is available for nucleic acids through our N•ESPript server.
ESPript is a utility, whose output is a PostScript / PDF / PNG or TIFF file of aligned sequences with graphical enhancements.
Its main input is a file of pre-aligned protein sequences in Clustal, FASTA, MultAlin, NPS@ or ProDom format.
The program calculates a similarity score for each residue of the aligned sequences.
Secondary structure elements can be added to the multiple sequence alignment using PDB, CIF or DSSP files.
A typical ESPript figure shows:
aligned sequences,
similarities,
consensus,
accessibility,
hydropathy,
secondary structure elements,
intermolecular contacts,
user-supplied markers.
Nice overview of ESPript and related programs by Dr. Brianna Bibel. For more engaging content on structural biology and biochemistry, visit her Bumbling Biochemist YouTube channel.
ENDscript is a friendly web server that automatically extracts and displays a comprehensive analysis of primary to quaternary protein structure information. Several ENDscript representations are produced using the ESPript core program.
NPS@ is an interactive web server dedicated to protein sequence analysis. NPS@ can perform multiple sequence alignments using ClustalW or MultAlin, and the resulting output can be displayed using ESPript.
ESPript is provided as a service to the scientific community and can be used free of charge for research and educational purposes without restriction.
We would appreciate it if you would use the appropriate citation when publishing data produced with ESPript.
Do not hesitate to contact us (espript@ibcp.fr) if you need any further information or if you need some help with ESPript.
The authors make no warranties regarding the correctness of the data. Their responsibility is limited to applying best efforts in providing the most reliable and accurate service. The authors are not responsible for the use of the results, data or information obtained from this server. This web server is hosted on the CNRS network and governed by French law. By accessing this website or using all or part of its functions, users formally accept the application of French law. This website does not use tracking cookies or cookies that collect personal data for marketing or analytical purposes (see our Cookie Policy). This website uses an encrypted connection (HTTPS). The IP address of the client computer and the date and time of the connection are processed for usage statistics. No other data is collected and no data is transferred to third party partners or sites.
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