ESPript  3.0

News  NEWS • September 9, 2014: if you want to relax, the ESPript 'Rebcross' game is online!
• July 1, 2014: ESPript3 paper is published in the 2014 Web Server Issue of Nucleic Acids Research (freely accessible online).
• January 23, 2014: T-Coffee is now interfaced with ESPript
• December 23, 2013: we are pleased to announce the release of a major update: ESPript version 3.0

Expand    Go to What is ESPript?
    Go to Terms of Use
What is ESPript?
  • ESPript, 'Easy Sequencing in PostScript', is a program which renders sequence similarities and secondary structure information from aligned sequences for analysis and publication purpose.

ESPript figure
Excerpt from a generated ESPript figure (full size in PDF)

  • Key features:

    • ESPript is a utility, whose output is a PostScript / PDF / PNG or TIFF file of aligned sequences with graphical enhancements.
    • Its main input is a file of pre-aligned sequences in Clustal, FASTA, MultAlin, NPS@ or ProDom format.
    • The program calculates a similarity score for each residue of the aligned sequences.
    • Optional files allow further rendering.

  • A typical ESPript figure shows:

    • aligned sequences,
    • similarities,
    • consensus,
    • accessibility,
    • hydropathy,
    • secondary structures elements,
    • intermolecular contacts,
    • user-supplied markers.

  • Biotool Web servers interfaced with ESPript:

    • ENDscript:
    • ENDscript is a friendly Web server, which extracts and renders a comprehensive analysis of primary to quaternary protein structure information in an automated way. Several ENDscript representations are produced with ESPript.

    • T-Coffee:
    • T-Coffee is a collection of tools for computing, evaluating and manipulating multiple alignments of DNA, RNA, protein sequences and structures.

    • NPS@:
    • NPS@ is an interactive Web server dedicated to protein sequence analysis. NPS@ can carry out multiple sequence alignments with ClustalW or MultAlin and resulting outputs can be rendered with ESPript.

    • MultAlin:
    • MultAlin is a multiple sequence alignment program with hierarchical clustering. Resulting alignments can be rendered with ESPript.

    • ProDom:
    • ProDom is a protein domain family database constructed automatically by clustering homologous segments. ESPript is used to print high-quality representations of domain families.

Google Scholar citations: ESPript 1 / ESPript 2 / ESPript 3

Terms of use
  • ESPript 3.x is developed and maintained by Patrice GOUET and Xavier ROBERT in the "Biocrystallography and Structural Biology of Therapeutic Targets" research team of the "Structural and Molecular Basis of Infectious Systems" laboratory (UMR5086 CNRS / Lyon University). ESPript 3.x is a major upgrade of ESPript 2.x, which was created by Patrice GOUET and Emmanuel COURCELLE.
  • ESPript is provided as a service to the scientific community and can be used free of charge for research and educational purposes without restriction.
  • When publishing data resulting from usage of ESPript, please use the following citation:
    • Robert, X. and Gouet, P. (2014) "Deciphering key features in protein structures with the new ENDscript server". Nucl. Acids Res. 42(W1), W320-W324 - doi: 10.1093/nar/gku316 (freely accessible online).
  • Send your messages to, if you have questions, suggestions or if you want to be informed about program releases.

SBGrid © 2005-2015 The ESPript authors & CNRS - Contact:
ESPript is an SBGrid supported application

Share ESPript in  Facebook Twitter Google Digg Reddit StumbleUpon Email